| Player | Residence | Position |
| Lindsay Addison | Platteville, WI | Scrum Half |
| Elise Anderson | River Falls, WI | Prop |
| Kaite Blunt | ||
| Leah Brandon | Wing, Center | |
| Sarah Brundidge | Marshfield, WI | Lock |
| Penny Bruskin | Bayside, WI | Hooker, Flanker |
| Meghan Connor | Troy, NY | Prop |
| Emily Crego | Coloma, WI | Wing |
| Anne David | Potosi, WI | Flyhalf |
| Nora Dinneen | Chicago, IL | Flanker |
| Cat Eskridge | Milwaukee, WI | Prop |
| Eliz Esikoff | Bridgewater, NJ | Fly Half |
| Ally Evans | Leicester, England | Center |
| Amanda Evenstone | West Allis, WI | Prop |
| Andrea Gall | Elkhorn, WI | Lock |
| Jayne Gonda | Lisle, IL | Lock |
| Elise Guthman | Mequon, WI | Hooker |
| Mary Hanks | Charlottesville, VA | Eight Man |
| Jenn Johnson | Hixton, WI | Scrum Half |
| Stormy-Kito Justice | Milwaukee, WI | Lock |
| Amy Kjedahl | Maple Grove, MN | Lock |
| Nikki Larmee | Evanston, IL | Prop |
| Andrea Larson | Plymouth, MN | Wing |
| Catie Mahon | Plymuth, MN | Inside Center |
| Megan Markielewski | Wing, Fullback | |
| Jee Minder | ||
| Amy Moorehouse | Scrumhalf | |
| Liz Nolan | Prop | |
| Johanna Nosal | Madison, WI | Scrum Half |
| Breta Olson | Youngstown, NY | Prop and Lock |
| Rachel Pagryzinski | Flanker | |
| Sarah Pebler | Hooker | |
| Gretchen Peterson | Wing, Center | |
| Maggie Perdzock | Pewaukee, WI | Center, Hooker |
| Ashley Pfeiffer | Wayzata, MN | Wing, Center |
| Kim Phillips | Bethesda, MD | Hooker/Flanker |
| Jessica Pizur | Muskego, WI | Eight Man |
| Jacquelyn Quintana | Wilmington, DE | Flanker, Lock |
| Kaite Raths | ||
| Hope Reddington | Waukesha, WI | Flanker |
| Jamie Rogalla | Wausau, WI | Prop |
| Kim Ruona | North Praire, WI | Flanker |
| Katie Schott | Madison, WI | Fullback |
| Meg Suess | Appleton, WI | Center |
| Brynn Van Hoomissen | New Prague, MN | Center |
| Emily Webb | Fredonia, WI | Flanker, Center |
| Rachael Weiker | Menomonie, WI | Lock |
| Sarah Weinberg | Evanston, IL | Center |
| Beth Whitaker | Milwaukee, WI | Back |
| Ingrid Wirts | Oconomowoc, WI | Center |
| Sara Zurn | Rosemount, MN | Full back |